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Novel Multiplex Assay Detects Citrus Pathogens

Technology Benefits
Increases uniformity and cost effectiveness User-friendly assay procedures Simultaneous detection, identification and quantification for the ten targets
Technology Application
Detection of endemic and exotic plant pathogens Plant health monitoring services Citrus disease management
Detailed Technology Description
None
Application No.
20160145678
Others

Images

Photo from Agricultural Research Service

Photo by Mmacbeth


Tech ID/UC Case

24385/2014-045-0


Related Cases

2014-045-0

*Abstract

Background:

Citrus greening disease, also known as huanglongbing (HLB), has been a serious, pervasive problem caused by a multitude of plant pathogens. It has decimated many citrus trees, drastically decreasing orange production and costing the US economy an estimated $11B every year. Currently, there is no cure for HLB, so the citrus industry is in dire need for a cost-effective method of early HLB detection.

 

Brief Description:

UCR Researchers have developed a means to detect and identify multiple plant pathogens for disease diagnosis, including citrus greening disease. By developing a novel multiplex RNA assay, they discovered ten targets of nine citrus pathogens and a citrus control gene. In addition to the assays, target-specific probes were designed and implemented to improve the pathogen detection process. These assays were also coupled with high-throughput robotic extraction and purification procedures, optimized for citrus tissues. Furthermore, they also developed a 3-plex DNA assay system along with 3 targets for simultaneous detection, identification and quantification of plant pathogens.

*Applications
  • Detection of endemic and exotic plant pathogens
  • Plant health monitoring services
  • Citrus disease management
*IP Issue Date
May 26, 2016
*Principal Investigator

Name: Jinbo Wang

Department:


Name: Georgios Vidalakis

Department:


Name: Tyler Dang

Department:

Country/Region
USA

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