New Collision-Induced Dissociation Cross Linker and Related Software Package for Fast and Accurate Mass Spectrometry Analysis of Proteins
- Technology Benefits
- This new cross-linker allows for the MS analysis of multi-subunit protein complexes. In addition, the accompanying software package allows for the fast and accurate identification of DSSO-cross-linked peptides via MS.
- Detailed Technology Description
- None
- Supplementary Information
- Patent Number: US20130144541A1
Application Number: US13471365A
Inventor: Rychnovsky, Scott | Huang, Lan
Priority Date: 14 May 2011
Priority Number: US20130144541A1
Application Date: 14 May 2012
Publication Date: 6 Jun 2013
IPC Current: G01N003368 | H01J004900
US Class: 702020 | 435023 | 546117 | 548259 | 548406 | 548462 | 548520
Title: MASS SPECTROMETRY-CLEAVABLE CROSS-LINKING AGENTS TO FACILITATE STRUCTURAL ANALYSIS OF PROTEINS AND PROTEIN COMPLEXES, AND METHOD OF USING SAME
Usefulness: MASS SPECTROMETRY-CLEAVABLE CROSS-LINKING AGENTS TO FACILITATE STRUCTURAL ANALYSIS OF PROTEINS AND PROTEIN COMPLEXES, AND METHOD OF USING SAME
Summary: (I) is useful for mapping protein-protein interactions of protein complexes and for integrated data analysis workflow for identification of crosslinked peptides (all claimed).
Novelty: New mass spectrometry cleavable crosslinker having two symmetric collision induced dissociation cleavable sites, useful for integrated data analysis workflow for identification of crosslinked peptides
- Industry
- Biomedical
- Sub Category
- DNA/Gene Engineering
- Application No.
- 9222943
- Others
-
State Of Development
This cross-linker and its accompanying software have been successfully used for the structural characterization of yeast 20 S proteasome complex and also demonstrated is identifying and characterizing model peptides and proteins.
Tech ID/UC Case
21327/2010-689-0
Related Cases
2010-689-0
- *Abstract
-
Researchers at the University of California, Irvine have developed a new and novel mass spectrometry (MS) cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for MS analysis of proteins. This DSSO cross-linker contains two symmetric collision-induced dissociation (CID) cleavable sites that allow for effective identification of DSSO-cross-linked peptides based on their distinct fragmentation patterns unique to cross-linking types such as interlink, intralink, and dead-ends.
A software package was also developed to be used with the DSSO cross linker. This software performs an integrated data analysis workflow for identifying DSSO-cross-linked peptides.
- *IP Issue Date
- Dec 29, 2015
- *Principal Investigator
-
Name: Lan Huang
Department:
Name: Scott Rychnovsky
Department:
- Country/Region
- USA
