Voltage-Sensitive Fluorescent (VF) Dyes For Neuronal Imaging
- Technology Benefits
- VF dyes add no capacitive load, making them ideal candidates for non-disruptive sensors of neuronal activityCapable of responding to rapid (millisecond) transmembrane potentials
- Technology Application
- Imaging of transmembrane events in neurons while providing a clear, readable signalSparse labeling of neurons with VF dyes while increasing the tractability of the approach for use in tissues, brain slices and in vivo contexts
- Detailed Technology Description
- None
- Application No.
- WO2016054442
- Others
-
Additional Technologies by these Inventors
- Long Wavelength Voltage Sensitive Dyes
- PHOTO-INDUCED ELECTRON TRANSFER VOLTAGE SENSITIVE DYES
- Voltage-Sensitive Dyes In Living Cells
- Xanthene-Based Dyes For Voltage Imaging
Tech ID/UC Case
24459/2015-044-0
Related Cases
2015-044-0
- *Abstract
-
The electrophysiological recordings of the activity of single neurons embedded within a network provides a powerful method for understanding brain function. While this approach has proven incredibly powerful, real limitations exist, namely the invasive requirement of sticking an electrode into biological samples, severely disrupting underlying tissue, restricting recordings primarily to cellular soma, and making recording from multiple sites challenging or impossible.
UC Berkeley researchers have developed compositions and methods for sparsely labeling neurons of cells with VF dyes. The VF dyes are sensitive to small variations in neuronal transmembrane potentials and can respond both to rapid and sustained membrane potential changes. The invention is also less susceptible to capacitative loading issues.
- *IP Issue Date
- Apr 7, 2016
- *Principal Investigator
-
Name: Vincent Grenier
Department:
Name: Evan Miller
Department:
- Country/Region
- USA
