A New Approach To Flow Cytometry, "nanocytometry"
- Technology Benefits
- Reproducibility, sensitivity, extremely small sample size.
- Technology Application
- Cancer diagnostics, cancer and stem cell research, clinical diagnostics.
- Detailed Technology Description
- None
- Supplementary Information
- Patent Number: WO2006124340A3
Application Number: WO2006US17485A
Inventor: SOHN, Lydia, L. | CARBONARIO, Andrea | GODLEY, Lucy, A.
Priority Date: 6 May 2005
Priority Number: WO2006124340A3
Application Date: 5 May 2006
Publication Date: 18 Sep 2008
IPC Current: G01N002700
Assignee Applicant: The Regents of the University of California
Title: MICROFLUIDIC SYSTEM FOR IDENTIFYING OR SIZING INDIVIDUAL PARTICLES PASSING THROUGH A CHANNEL | SYSTEME MICROFLUIDIQUE PERMETTANT D'IDENTIFIER OU DE CALIBRER DES PARTICULES INDIVIDUELLES CIRCULANT DANS UN CANAL
Usefulness: MICROFLUIDIC SYSTEM FOR IDENTIFYING OR SIZING INDIVIDUAL PARTICLES PASSING THROUGH A CHANNEL | SYSTEME MICROFLUIDIQUE PERMETTANT D'IDENTIFIER OU DE CALIBRER DES PARTICULES INDIVIDUELLES CIRCULANT DANS UN CANAL
Summary: The apparatus is useful for identifying individual particles, e.g. cell; cell fragment; colloid; or bacterium, virus, fungus, micelle, liposome, DNA or RNA or an oligonucleotide chain (claimed); and useful for characterization of cancer and other types of cells; to measure patient responses to cytotoxic chemotherapy; or isolation of circulating tumor and/or endothelial cells from patients with solid tumors and/or isolation of circulating hematopoietic precursor cells from patients.
Novelty: Individual particles identifying apparatus useful, e.g. for characterization of cancer and other types of cells, comprises measuring system to measure period during which particle moves through channel
- Industry
- Disease Diagnostic/Treatment
- Sub Category
- Cancer/Tumor
- Others
-
Tech ID/UC Case
17511/2005-075-0
Related Cases
2005-075-0
- *Abstract
-
Conventional flow cytometry has made valuable contributions to cancer diagnosis and management as well as to the understanding of fundamental cancer cell biology. Flow cytometry is used routinely in the clinical diagnosis of the hematologic malignancies; in tumor immunology to define lymphocyte subsets; and in basic research to facilitate cell separations based on the expression of particular proteins or phospholipids at the cell-surface. However, it does require a large sample of cells and usually requires labeling.
Researchers at the University of California, Berkeley have developed a new approach to flow cytometry; the researchers call it "NanoCytomerty." The novel technology uses an integrated microfluidic chip which can adapt to sort cancer and other types of cells based on their cell-surface protein expression. The technology allows for significant improvements over conventional flow cytometry, because the system permits label free signal detection, extreme reproducibility and sensitivity, and cell separations using very few cells.
By developing a more sensitive technique to perform cell separations, in addition to one that relies on fewer cells, we anticipate that NanoCytometry could provide an important new technology applicable to cancer. For instance, NanoCytometry could be used to improve upon physicians' ability to detect minimal residual disease states and upon a scientist's ability to study cell populations that occur in very small numbers such as stem cells. Nanocytometry builds upon previous work which includes an all-electronic technique for detecting the binding of unlabeled antibody-antigen pairs (US Patent Appl. # 10/056,103).
- *Principal Investigator
-
Name: Andrea Carbonaro
Department:
Name: Lucy Godley
Department:
Name: Lydia Lee Sohn
Department:
- Country/Region
- USA
