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In Vitro Mesangial Cell Model for Progression of Glomerulosclerosis

Detailed Technology Description

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*Abstract

Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements. An in vitro kidney mesangial cell model has been produced for the investigation of mechanisms by which glomerulosclerosis develops in non-diabetic kidney disease. Researchers have characterized the mesangial cells as high producers of extracellular matrix proteins by isolating glomerular mesangial cells from a mutant mouse model (Fvb Os/+ mice with ANAPC-10 gene mutation) of progressive, non-diabetic glomerulosclerosis and severe renal failure. The growth characteristics of the cell line have been characterized and some pathways were activated in these cells that likely contribute to the persistent, high matrix-producing phenotype. This cell line will be useful in examining the mechanisms by which excess matrix production leads to glomerulosclerosis and to test new drugs that may inhibit this scarring process.

*Principal Investigator

Name: Charles Heilig

Department:

Name: Kathleen Heilig

Department:

Country/Region

USA