B-13 Tol1 Gene Transfer System
- Technology Benefits
- ΓùÅ Tol1 is a DNA-based transposable element. Simplicity and safety of usage in comparison with RNA-mediated elements and retroviruses. ΓùÅ High Capacity; maximum cargo capacity is >20kb.ΓùÅ Universal use: available for fish, frog and mammalian cells, such as mouse, human and possibly also for other mammals.
- Technology Application
- Biological Tool for gene engineering and gene therapy.
- Detailed Technology Description
- The Tol1 gene transfer system consists of two plasmids. One is Tol1 donor (pDon) and the other is transposase plasmid (pHel). For the generation of founder transgenic fish, pDon and pHel are co-injected into fish or frog embryos or mammalian cells.
- Supplementary Information
- Patent Number: WO2008072540A1
Application Number: WO2007JP73565A
Inventor: KOGA, Akihiko | HAMAGUCHI, Satoshi
Priority Date: 13 Dec 2006
Priority Number: WO2008072540A1
Application Date: 6 Dec 2007
Publication Date: 19 Jun 2008
IPC Current: C12N001509 | C12N000510 | C12N000910
Assignee Applicant: National University Corporation Nagoya University
Title: Tol1 FACTOR TRANSPOSASE AND DNA INTRODUCTION SYSTEM USING THE SAME | TRANSPOSASE DE FACTEUR TOL1 ET SYSTÈME D'INTRODUCTION D'ADN UTILISANT CELLE-CI
Usefulness: Tol1 FACTOR TRANSPOSASE AND DNA INTRODUCTION SYSTEM USING THE SAME | TRANSPOSASE DE FACTEUR TOL1 ET SYSTÈME D'INTRODUCTION D'ADN UTILISANT CELLE-CI
Summary: The Tol1 factor transposase is useful for introducing DNA of Tol1 factor which is useful in gene therapy.
Novelty: Novel transposable element of Oryzias latipes 1 Tol1 factor transposase having enzyme activity for transferring Tol1 factor, for introducing DNA of Tol1 factor useful in gene therapy
- Industry
- Biomedical
- Sub Category
- Medical Device
- *Abstract
-
Researchers at Nagoya University discovered a new transposon named Tol1 from medaka fish, which is a powerful tool for basic and traslational molecular biology applications.
- *Principal Investigator
-
Name: Akihiko Koga, PhD, Professor
Department: Primate Research Institute, Kyoto University
- Country/Region
- USA
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