Expanding ChIPΓÇÖs Reach: Automating ChIP for High-throughput Screens and ChIP Controls to Increase Sample Comparability
- Detailed Technology Description
- None
- *Abstract
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Current ChIP tools suffer from low throughput, lack of reproducibility, and difficult sample to sample comparisons. However, scientists at the Broad Institute have developed ChIP methods through the construction and purification of a sequencing library that not only show superior yield, comparability and sensitivity to trace proteins, but can be fully automated for high throughput screening ΓÇöan improvement previously blocked by the hands-on time required for existing ChIP protocols. The improvements open the door to scaled-up use of ChIP to screen large numbers of samples in parallel. This advancement will enable better chromatin-protein mapping and improve the opportunity to develop therapeutic and diagnostic programs. Further expanding ChIPΓÇÖs reach, Broad scientists have also developed control agents for ChIP assays. These agents are a collection of peptide-oligo conjugates which can be annealed into the experiment and undergo all the process steps in parallel to the genomic material. Sequencing information on these conjugates in addition to the standard ChIP-Seq data can be used to normalize ChIP and other assay data.
- Country/Region
- USA
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