Multiflavor Streptavidin
- Detailed Technology Description
- None
- *Abstract
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Compounds and methods are described for producing streptavidin mutants with greater affinity for biotin substitutes than for biotin. The compounds and methods of the present invention are particularly useful where levels of endogenous biotin are present in the system, precluding the use of the standard biotin-avidin approach. In addition, it is contemplated that the streptavidin-biotin system can be used as a model to test if the contacts that exist between a protein and a ligand can serve as the starting point to genetically engineer the protein to develop a high specificity for another ligand. The strategy is contemplated to be useful to develop a receptor for a molecule without a known receptor when phage-display methodologies cannot be employed, such as in the case of a multi-chain protein, for the discovery of new drugs and diagnostic reagents, or in applications where the use of one molecule is well-suited for a project but the other one is not.
- Country/Region
- USA
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