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The ability of prokaryotic organisms to survive and develop single and multi-drug resistance has been observed from the earliest use of the first antimicrobial agents. This invention provides a simple genetic system with rRNA genes that can elucidate the molecular mechanism of translation and would..... [Detail]
Applications This plasmid expresses the fusion gene CAS9-HA-2xNS-GFP (S. pyogenes Cas9 sequence with atwice-repeated sequence of the simian virus 40 nuclear localization signal andGFP) in the pTREX-n backbone. This vector is used for cloning a specific sgRNAby BamHI, to be co-expressed with Cas9 for..... [Detail]
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