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Click-Mass Spectrometry of lipids

總結: Click-Mass spectrometry of lipids via bicyclic triazolium ions lead to predictable fragmentation with promising Multiplex analysis.

技術優勢: Higher sensitivity as comparable analysis procedures
Fast detection method for tracing of fatty acid metabolism without usage of radioisotopes
Hundreds of labelled species detected by automated analysis of primary MS data
Simple absolute quantification by internal standardization
Combination of any other analysis method possible
High stability of bicyclic Triazolium ions leads to predictable fragmentation
Heavy-atom labelled compounds enable Multiplex analysis

技術應用: studying lipid metabolism, Understand and detect diseases such as metabolic syndrome or type 2 diabetes.

詳細技術說明: The present innovative click-chemistry-based method allows tracing of fatty acid metabolism in virtually any biological system. It combines high sensitivity with excellent linearity and fast sample turnover. Since it is free of radioactivity, it can be combined with any other modern analysis technology and can be used in high-throughput applications.
In an improved and preferred method, multiplexing of samples can be carried out. After the labeling reaction, samples are combined for MS analysis. This increases the sample turnover and improves comparison by reducing stochastic experimental variations.

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