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New Resins for Serial Block-Face Scanning Electron Microscopy

詳細技術說明: University researchers have developed new resins to immobilize tissue samples in Serial Block-face Scanning Electron Microscopy (SBFSEM). The invention has been demonstrated to lead to dramatic improvements in image contrast and resolution for SBFSEM.

*Abstract: The advent of serial block-face scanning electron microscopy (SBFSEM) promises to revolutionize histology and neuroanatomical research by allowing the 3-dimensional reconstruction of relatively large regions of tissue and cell arrays at near nanometer-scale resolution. In SBFSEM, successive slices are removed from the targeted tissue and an electron beam is scanned over the remaining block-face to produce electron backscatter images. A principal limitation of this approach is that the resolution obtainable using backscatter electron imaging at low accelerating voltage is modest compared to traditional transmission electron microscopy.

*Principal Investigation: Name: James Bouwer
Department:

Name: Eric Bushong
Department:

Name: Thomas Deerinck
Department:

Name: Mark Ellisman
Department:

Name: Donald Johnson
Department:

Name: Ranjan Ramachandra
Department:

Name: Jay Siegel
Department:

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