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B-12 Fish Transplantation Technology

技术优势

ΓùÅ An easy nuclear transplantation method for fishΓùÅ Low cost, minimum labor to create transgenic fishΓùÅ Low cost to create fish gene library

技术应用

ΓùÅ Cost and time effective transgenic fish productionΓùÅ Establishment of cost-effective storage system for commercial or endanger fish

详细技术说明

The step of preparing a fish embryo in this new method comprises a step of imposing physical and/or chemical stress to the unfertilized egg after activation at early stage of the development stages. Because a nuclear transplantation is applied to recipient eggs during its G2 or M developing stage, the correct embryonic chromosomal ploidy can be secured and transgenic fish can be produced more efficiently. This technique is also available to use as a new storage system for the establishment of valuable fish libraries.

*Abstract

Researchers at Nagoya University succeeded in circumventing this difficulty by using a new nuclear transfer method in medaka fish (Oryzias latipes, aka Japanese Killifish.) 1. Unfertilized eggs from recipient fish are subjected to heat-shock treatment.2. Donor cells were prepared from primary caudal fin cultures from single adult fish.3. Donor cell injected to heat-treated eggs by microinjection.

附加资料

Inventor: WAKAMATSU, Yuko | OZATO, Kenjiro
Priority Number: WO2007096985A1
IPC Current: A01K006702 | A01K0067027 | C12N000510 | C12N001509
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR PREPARING FISH EMBRYO | PROCEDE DE PREPARATION D'UN EMBRYON DE POISSON
Usefulness: METHOD FOR PREPARING FISH EMBRYO | PROCEDE DE PREPARATION D'UN EMBRYON DE POISSON
Summary: For producing fish embryo, which is useful for producing transgenic fish such as Japanese killifish ( Oryzias latipes ) (all claimed), goldfish, zebra fish, salmon, carp, flatfish, eel, yellowtail, trout and sea bream. The transgenic fish is useful as ornamental fish, and as model for diagnosis, gene expression analysis, drug development and environment assessment, for environmental monitoring of various disease and environmental hormone equalization studies.
Novelty: Preparing fish embryo for producing transgenic fish e.g. Japanese killifish and salmon, by subjecting activated unfertilized egg to physical/chemical stress, transducing nucleus of fish to unfertilized egg and producing fish embryo

主要类别

农业

细分类别

植物

国家/地区

美国