Gene Editing: An Improved Methodology For Homology Directed Repair In Cells
- Technology Benefits
- This new invention has been found to increase the HDR efficiency by 2- to 15-fold
- Technology Application
- The invention has wide applications including use in treatment of human genetic diseases as well as performing genetic engineering on cells or organisms with the goal of producing better pharmaceuticals.
- Detailed Technology Description
- Researchers at UC San Diego have developed a method to increase the efficiency of homology directed repair (HDR). This method uses CRISPR-Cas9 technology to generate the DNA double-strand breaks, and then employs a novel approach for HDR. In some instances, this method also decreases the amount of cell death that occurs during the HDR process.
- Others
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State Of Development
A working research model.
Intellectual Property Info
This technology is patent pending and available for licensing and/or research sponsorship.
Tech ID/UC Case
29620/2018-298-0
Related Cases
2018-298-0
- *Abstract
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Gene editing in cells involves the use of sequence-specific nucleases that generate double-strand DNA breaks (DSBs) at specifically targeted sites in the genome. The DSBs are then repaired by non-homologous end joining (NHEJ) or, much less efficiently, by homology directed repair (HDR). In NHEJ, the two free DNA ends are joined together. This commonly results in small DNA deletions or insertions that inactivate the target gene. In HDR, the DSB is repaired by a mechanism that converts the DNA into the specific sequence that is provided by a DNA donor template. Thus, HDR enables genes to be modified or inserted at specifically desired sites. The main drawback to HDR is the low efficiency of the process.
- *Principal Investigator
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Name: Grisel Cruz Becerra
Department:
Name: James Kadonaga
Department:
- Country/Region
- USA
