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Method to Quantify Exosome Concentration

Technology Benefits

Simple and affordable method forexosome concentration quantificationExosome detection in extremely smallsample volumesPotential for use in cell culture,animal models, or other in vivosystems to localize and quantify exosomesApplication in laboratories, researchtools, and exosome characterization such as inflammation, immunotherapy, aging,and anti-tumor effects

Detailed Technology Description

A new technique for quantifying exosomeconcentration.

*Abstract

With the use of specially formulated, inexpensivenanoparticles, this technology provides an economical method for quantifyingexosomes in extremely small sample volumes (~1µL). Additionally, the exosomecompounds may be quantified or localized using magnetic resonance imaging (MRI).This invention could not only make quantifyingexosome concentration faster and more economical, but also serve as a firststep in exosome characterization protocol in these developing applications.

*Background

Exosomesare extracellular vesicles that are reported to be potential diagnostic markersfor health disorders, and new studies show they may have a use in drug-deliveryand gene-therapy applications. Currently, several different methods are used totry to quantify exosome concentration. Some methods are directed to the use ofprotein assay (such as a Bradford Assay) to quantify exosome concentration;however, the use of a protein assay is not a direct measurement of exosomessince exosomes are individual particles and the protein content of a particlecan differ. Other methods, such as protein assays or nanodrop spectrophotometry,only determine the total protein in a sample, and Dynamic Light Scattering(DLS) only givesparticle diameters and population information. Furthermore, NanoparticleTracking Analysis (NTA) systems, which are most commonly used to quantifyexosome concentration, are far too expensive, limiting its applicability forthe average research laboratory. Thus, there is a present market need forfast and inexpensive methods of quantifying exosome concentration in culturemedia and body fluids.

*Inquiry

STC has filed intellectual property on this exciting new technology and is currently exploring commercialization options. If you are interested in information about this or other technologies, please contact Arlene Mirabal at amirabal@stc.unm.edu or 505-272-7886.

Country/Region

USA