Efficient Library Preparation for CRISPR Pooled Single-Guide RNAs Screens
- Technology Application
- This new technology is platform and library agnostic, and provides a dramatic improvement for all such approaches by simplifying and improving library preparation, enabling larger scale studies, higher reproducibility, and higher sensitivity in identifying candidates for further study.
- Detailed Technology Description
- Researchers from UC San Diego have developed a method to enable the generation of high-throughput sequencing libraries from DNA isolated from a population of cells containing a pooled library of gene targeting constructs.
- Others
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Intellectual Property Info
A provisional patent has been submitted and the technology is available for licensing
Tech ID/UC Case
29346/2017-105-0
Related Cases
2017-105-0
- *Abstract
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There is great interest in both academic and commercial labs in performing pooled CRISPR screens for a variety of purposes, including identifying drug resistance and delivery mechanisms, genes essential for survival, death and disease phenotypes, differentiation, regulation of gene expression, and various other mechanisms.
- *Principal Investigator
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Name: Alexander Shishkin
Department:
Name: Eric Van Nostrand
Department:
Name: Eugene (Gene) Yeo
Department:
- Country/Region
- USA
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