Anti-Feline Proinsulin and Insulin Monoclonal Antibodies (10-line panel)
- Detailed Technology Description
- ApplicationsAnti-felineproinsulin antibodies useful for the study of feline metabolism and beta cellfunction. Possible applicability in the development of clinical diagnosticassays. Used in the development ELISA assays. Proinsulin is the precursor tothe prohormone, insulin, as well as the protein C-peptide made from beta cellsin the pancreas. Mature insulin has 35fewer amino acids than proinsulin, 4 are removed altogether and the remaining31 form the C-peptide. The C-peptide is abstracted from the center of theproinsulin sequence and the two other ends (the B chain and A chain) remainconnected by disulfide bonds. The common spontaneous form of diabetes mellitusseen in cats closely resembles that of human type 2 diabetes. Similar riskfactors for the disease are seen in cats and people, such as age and obesity.Progression to type 2 diabetes is marked by increased resistance to insulinaction in peripheral tissues, especially skeletal muscle and adipose tissue,and decreased function of the ß-cell in insulin production and/or secretion.Early detection of ß-cell failure is critical in determining therapeutic routesto counter the disease. Recently, in human studies, it has been found that theearliest stages of ß-cell function loss can be observed as an increase in theproinsulin/insulin ratio in serum during fasting states. These antibodies whosespecificity for feline insulin, proinsulin, and C-peptide may prove useful inthe development of highly specific assays for determining serum concentrationsof intact feline proinsulin and insulin.
- *Abstract
-
ReagentDescription
Antigen:
Proinsulin
Accession ID:
Q52PU3 (cat pre-proinsulin)
Clonality:
Monoclonal
Clone Name:
11E6.A5 (IgG1, B-chain); 4H2.A8 (IgG2b); 7H3.A2 (IgG1, B-chain); 2E4.C2 (IgG1, PC1), 4E11.A2.C3 (IgG1, B-chain); 8E7.B12 (IgG2b, PC1 and PC2); 4F12.F2 (IgG1, PC1); 6G3.F1 (IgG1, C-pep); 3C11.F1 (IgG1)
Reactivity:
Cat
Immunogen:
Protein
Species Immunized:
Mouse
Epitope:
Pro-insulin (B-chain, PC1, PC2, C-peptide)
Purification Method:
Protein G
Buffer:
0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications:
IRMA, ELISA
References
Caffall, ZF.Development of monoclonal and polyclonal antibodies against feline proinsulinand C-peptide. MS Thesis. The Universityof Georgia, Athens, GA (2006).
Kley S, CaffallZ, Tittle E, Ferguson DC, Hoenig M. Development of a feline proinsulinenzyme-linked immunosorbent assay (ELISA): A novel application to examine betacell function in cats. Domest AnimEndocrinol. 2008 Apr;34(3):311-8.
Hoenig M, TraasAM, Schaeffer DJ. Evaluation of routine hematology profile results andfructosamine, tyroxine, insulin, and proinsulin concentrations in lean,overweight, obese and diabetic cats. J Am Vet MedAssoc. 2013 Nov 1;243(9):1302-9.
- Country/Region
- USA
