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Anti- Lipoprotein I (LpI) antibody panel (5 lines)

Detailed Technology Description: Applications RecognizeCallinectes sapidus (blue crab) (andpossibly multiple other crustaceans) Crustacean LpI. May react with a broaderspectrum of Lps from crustaceans and may be especially useful in the study ofcrustaceans immunologicalresponse to, e.g., environmental stress, polution and diseases. Suitable for Immunofluorescence, Western Blotand ELISA applications. Useful in ecology and marine biology. A lipoprotein is a biochemical assembly whose purpose is totransport hydrophobic lipid molecules in water, as in blood or ECF.Specifically, LPI is in the hemolymph of crustaceans and transports lipid toperipheral tissues and also has a role in crustacean immune recognition. LpIwas first found in stage 5 embryos and appeared to be synthesized by lateralbasophilic cuboidal cells that demonstrated cytoplasmic immunoreactivity forLpI at their interface with the yolk mass. The embryonic cuboidal cells bore astrong cytologic resemblance to the hepatopancreas cells of later stages (zoea,megalopae, adults), which were also immunoreactive for LpI.

*Abstract

ReagentsDescription

Clone Name	Isotype	Protein MW (kDa)	Reactivity
11A8.A7	IgM	224	Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine invertebrates
14G5.G12	IgM	224	Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine inverterbrates
4H2.C6	IgM	224	Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine inverterbrates
9A11.B5	IgM	224	Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine inverterbrates
9F5.D12	IgM	224	Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine inverterbrates

Antigen:Crustacean Lipoprotein I (LpI)

Immunogen: Lipoprotein

Species Immunized: Mouse

Purification Method: used as supernatant

Validated applications: ELISA, WB, IF

Antibody storage temperature: -80^oC

References

Walker A, AndoS, Lee RF. Synthesis of a high-density lipoprotein in the developing blue crab(Callinectes sapidus). Biol Bull. 2003Feb;204(1):50-6.

Walker A, LeeRF. Lipovitellin and Lipid Droplet Accumulation in Oocytes During OvarianMaturation in the Blue Crab, Callinectes sapidus. ExperimentalZoology. 1995: 271:401-412

Country/Region

USA

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