Anti- E. coli LacI (lac repressor) monoclonal antibodies (2 lines)
- Detailed Technology Description
- Applications Suitablefor WB. Lac repressor (LacI) is a DNA-binding protein which inhibitsthe expression of genes coding for proteins involved in the metabolism oflactose in bacteria. In the absence of lactose, the Lac repressor binds to theoperator and keeps RNA polymerase from transcribing the Lac genes. Reacts with native and recombinant LacI expressed in E. coli or Arabidopsis. Predicted to react with multiple other species (seetable).
- *Abstract
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ReagentsDescription
Clone Name
Accession #
Isotype
Protein MW
(kDa)
Reactivity
15C1
AY042185
IgG1
45
E. coli and Arabidopsis.
Possibly: Actinopolyspora erythraea, A. algeriensis, Chlamydia trachomatis, C. muridarum, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
17D7
AY042185
IgG1
45
E. coli and Arabidopsis
Possibly: Actinopolyspora erythraea, A. algeriensis, Chlamydia trachomatis, C. muridarum, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
Antigen:LacI
Immunogen: Recombinant LacI-NLS-His6made in E. coli
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl,0.05% (w/v)
Sodium Azide
Validated applications: WB (5 μg in 10 mL, 0.5 μg/mL)
Antibody storage temperature: -20oC
References
KimT, Balish RS, Heaton AC, McKinney EC, Dhankher OP, Meagher RB. Engineering aroot-specific, repressor-operator gene complex. Plant BiotechnolJ. 2005 Nov;3(6):571-82.
- Country/Region
- USA
