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Novel Biosensor for the Diagnosis of Cervical and Other Cancers

Technology Application

Diagnosis of cervical cancer with Pap test samples as well as other types of cancersCombining with a standard Pap test to reduce the false-negative rate of cervical cancer diagnosisScreening of new anticancer drug candidates against cervical cancer at different developmental stages

Detailed Technology Description

Src is a key molecule in oncogenesis and plays a major role in early development and progression of cervical and other types of cancer such as breast, colon, pancreatic, and lung. The novel biosensor developed by UCSD researchers combines FRET technology with genetically encoded biosensors which allows the monitoring of the Src tyrosine kinase activity in live cells, with high temporal and spatial resolutions. This innovation can potentially improve the sensitivity and accuracy for diagnosis of cervical cancer with Pap test samples.

Supplementary Information

Patent Number: US8114581B2
Application Number: US2007992031A
Inventor: Chien, Shu | Wang, Yingxiao
Priority Date: 15 Sep 2005
Priority Number: US8114581B2
Application Date: 14 Mar 2008
Publication Date: 14 Feb 2012
IPC Current: C12Q000100 | C07H002104 | C12N001500
US Class: 435004 | 4353201 | 5360234
Assignee Applicant: The Regents of the University of California
Title: Methods and compositions for detecting neoplastic cells
Usefulness: Methods and compositions for detecting neoplastic cells
Summary: The polypeptide is useful in identifying a neoplastic cell in a biological sample.
Novelty: New chimeric polypeptide comprising a membrane translocation specific, a first fluorescent, a Src homology, a phosphorylatable substrate or a second fluorescent domain, useful in identifying a neoplastic cell in a biological sample

Industry

Disease Diagnostic/Treatment

Sub Category

HIV

Application No.

8114581

Others

State Of Development

This technology has been further improved in the biosensor sensitivity, validated in cell culture and cervical biopsy samples.

Other Information

UCSD Researcher:

Dr. Chien, M.D., Ph.D., is University Professor and Director of the UCSD Whitaker Institute of Biomedical Engineering and former chair of the Jacobs School of Engineering’s, Department of Bioengineering.

Web site

Lab 

 

Related Materials

Wang Y, Botvinick EL, Zhao Y, Berns MW, Usami S, Tsien RY, Chien S. Visualizing the mechanical activation of Src. Nature. 2005 Apr 21; 434 (7036):1040-5.
Wang Y, Chien S. Analysis of integrin signaling by fluorescence resonance energy transfer. Methods Enzymol. 2007; 426: 177-201.
Vascular Bioengineering Laboratory
UCSD Bioengineer Shu Chien Accepts Lifetime Achievement Award
Ouyang M., Sun J., Chien S., Wang Y. (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors, Proc Natl Acad Sci USA. 105 (38):14353-58

Tech ID/UC Case

19813/2005-089-0

Related Cases

2005-089-0

*Abstract

Carcinoma of cervix is the major malignancy of women. For cervical cancer diagnostics, there are about 55-million Pap smear tests performed every year in the United States. Pap smear screening for cervical cancer can result in 20% to 40% of false-negative results that lead to sometimes painful biopsies and put women at risk for pregnancy complications such as preterm labor and low-birth weight infants. Therefore there is a great need for a more accurate test of developmental stages of cervical cancers.

*IP Issue Date

Feb 14, 2012

*Principal Investigator

Name: Shu Chien

Department:

Name: Yingxiao Wang

Department:

Country/Region

USA