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A Novel Technique for Fabricating Biomolecular Nano-Arrays Enabling High-DNA Amplification and Sequencing

Technology Application

This technology may be applied to high-throughput genome sequencing, protein array analysis, and bio-sensors.

Detailed Technology Description

This invention provides a new approach for fabricating high-density nano-arrays for bio-molecules. Researchers used glass slides derivatized with functional groups, then coated them with a thin layer of photo-resist. Conventional photolithography is then used to create high-density wells of sub-micron dimension into which nano-particles conjugated to a single DNA clone are allowed to self-assemble. The small size of the wells prevents more than one molecule from attaching in any given well. The nano-particles remain fixed in their wells through biotin-avidin with the remaining non-specifically bound nano-particles being washed away with the removal of the remaining photo-resist. This leaves a very clean array for high throughput sequencing.

Supplementary Information

Patent Number: US20100120630A1
Application Number: US2009525068A
Inventor: Huang, Xiaohua | Barbee, Kristopher D.
Priority Date: 30 Jan 2007
Priority Number: US20100120630A1
Application Date: 29 Dec 2009
Publication Date: 13 May 2010
IPC Current: C40B004000 | C40B005018 | C40B006014
US Class: 506013 | 506032 | 506040
Assignee Applicant: The Regents of the University of California
Title: METHODS AND DEVICES FOR BIOMOLECULAR ARRAYS
Usefulness: METHODS AND DEVICES FOR BIOMOLECULAR ARRAYS
Summary: A high density biomolecular array including protein arrays for chip-based analysis, and DNA arrays for genotyping or genome sequencing.
Novelty: Formation of high density biomolecular array e.g. DNA arrays for genome sequencing, by patterning array of wells into photoresist on surface, forming suspension of particles, immobilizing particles to surface, and removing unbound particles

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

9063133

Others

Other Information

This invention has a patent pending.

Additional Technologies by these Inventors

• Methods and Systems for Direct Sequencing of Single DNA Molecules
• Methods And Apparatuses For Duplicating Genomic Dna With Contiguity Barcodes For De Novo Genome And Epigenome Sequencing

Tech ID/UC Case

19180/2007-085-0

Related Cases

2007-085-0

*Abstract

None

*IP Issue Date

Jun 23, 2015

*Principal Investigator

Name: Kristopher Barbee

Department:

Name: Xiaohua Huang

Department:

Country/Region

USA