Monoclonal Antibodies Against Chk2 (Clone 4B8)
Specific antibody to detect Chk2 by antibody-antigen complex formation.
Immunoprecipitation Western blot
Chk2 is a member of the CDS1 subfamily of serine/threonine protein kinases. Chk2 is a cell cycle checkpoint regulator and putative tumor suppressor. A forkhead-associated protein interaction domain is critical for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Once activated, Chk2 inhibits CDC25C phosphatase, preventing mitosis, and has been shown to stabilize the tumor suppressor protein p53. Chk2 also interacts and phosphorylates BRCA1 to promote cell survival after DNA damage. Mutations in Chk2 are linked to Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype typically associated with inherited mutations in TP53. These mutations are also thought to confer predisposition to other types of cancer. The described mouse monoclonal IgG1 antibody binds to human Chk2 and has been described for use in immunoprecipitation and western blot.
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