Long Wavelength Voltage Sensitive Dyes
- Technology Benefits
- Can be interfaced with a wide variety of optical toolsCan be used in a wide range of biological contextsHigh spatial resolution, high throughput, minimally invasiveExcellent photostability
- Technology Application
- Optically track action potentials in neuronsScreen drug interactions and complications with heart ion channels
- Detailed Technology Description
- None
- Others
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Additional Technologies by these Inventors
- Voltage-Sensitive Fluorescent (VF) Dyes For Neuronal Imaging
- PHOTO-INDUCED ELECTRON TRANSFER VOLTAGE SENSITIVE DYES
- Voltage-Sensitive Dyes In Living Cells
- Xanthene-Based Dyes For Voltage Imaging
Tech ID/UC Case
25104/2015-186-0
Related Cases
2015-186-0
- *Abstract
-
Rapid changes in the membrane potential of neurons and cardiomyocytes define the unique physiology of these cells. The classical means to monitor membrane potentials is patch clamp electrophysiology, a low-throughput and highly invasive technique. One current alternative is to use calcium imaging, as the agents are robust and sensitive, come in a variety of colors, and can be used in a wide range of biological contexts. Calcium imaging, however, allows only an imperfect approximation of membrane potential changes, and fast-spiking neuronal events are difficult to resolve.
Fluorescent voltage sensors can achieve fast, sensitive, and non-disruptive direct readouts of membrane potentials. UC Berkeley researchers have designed and synthesized a new fluorophore called ‘Berkeley Red’ that can be used in the context of voltage-sensing scaffolds to generate fluorescent voltage sensors.
- *Principal Investigator
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Name: Yi Lin Huang
Department:
Name: Evan Miller
Department:
Name: Alison Walker
Department:
- Country/Region
- USA
