Separation system with a sheath-flow supported electrochemical detector
Electroactive analytes can be transported to an EC detector placed relatively far away from the end of a CE separation column providing decoupling of the CE electric field from the EC detectorDetector sensitivity is significantly increased while separation efficiency is enhanced. Larger separation channels and higher separation electric fields can also be usedEC detector is reliable and relatively easy to use
Gel electrophoretic separation of DNAMultiplex electrochemical detectionSingle nucleotide polymorphism (SNP) genotyping
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Patent Number: US7438792B2
Application Number: US2005107295A
Inventor: Mathies, Richard A. | Emrich, Charles A. | Singhal, Pankaj | Ertl, Peter
Priority Date: 20 Apr 2004
Priority Number: US7438792B2
Application Date: 14 Apr 2005
Publication Date: 21 Oct 2008
IPC Current: G01N0027447 | G01N002727 | G01N0027453 | G01N002749
US Class: 204452 | 204409 | 204603
Assignee Applicant: The Regents of the University of California
Title: Separation system with a sheath-flow supported electrochemical detector
Usefulness: Separation system with a sheath-flow supported electrochemical detector
Summary: For chip-based separation.
Novelty: Electrochemical detector for chip-based separation, comprises first and second channels that provide sheath flow at juncture of first and second channels with exit end of separation channel
Measurement/Testing
Measurement Platform
Brief Description High-speed electrophoretic separations of biomolecules are now routinely performed in micron-sized capillaries and have been proven on microfabricated capillary electrophoresis (CE) devices. These techniques offer a significant increase in speed over previous techniques. However, the fluorescence-based detection methods often employed require large and expensive optical detection systems. Significant cost and space savings can be achieved by using electrochemical (EC) methods for detection. An important issue when performing amperometric detection with CE is the necessary electrical isolation of the EC detector from the separation voltage, since interference from the CE current significantly influences detector performance. Publications Capillary electrophoresis chips with a sheath-flow supported electrochemical detection system Additional Technologies by these Inventors Tech ID/UC Case 17407/2004-100-0 Related Cases 2004-100-0
UC Scientists described an EC detector that reduces background currents, has increased sensitivity and enhances the separation efficiency of CE systems. The microfabricated sheath-flow EC detector controls and minimizes interference from EC potentials. The precise control of critical electrode characteristics, such as shape, size and orientation, on a microchip makes microfabrication an attractive tool for the development of EC detection systems. DNA fragment sizing was demonstrated using the redox-active intercalator dye iron phenanthroline. SNP genotyping using allele-specific PCR was successfully accomplished using specific ferrocene-labeled primers. This sheath-flow supported CE-EC system permits the use of multiple electrode systems capable of analyzing complex analyte mixtures, and truly portable microfluidic device and micro-total analysis systems.
USA
