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Second harmonic generation for measuring the relative orientation in a pair of DNA-binding molecules

Summary

Kenneth Eisenthal, Ph.D.

Technology Benefits

Measures drug-induced DNA unwinding.Acquires data in solution more closely mimicking the DNA environment in vivo.Improves research and development of new DNA-binding drug candidates.Patent information:Patent Pending (US/2012/0202296)Tech Ventures Reference: IR CU13153

Technology Application

Screen the effects of DNA-binding compounds on DNA unwinding.Probe basic structural features of biochemical complexes for the study of structural biology.Provide information on other potential structural changes induced by drug binding distinct from DNA unwinding, such as kinking or supercoiling.Use as a general DNA binding assay if SHG intensity reproducibly changes when a compound binds to DNA.

Detailed Technology Description

Kenneth Eisenthal, Ph.D.

*Abstract

None

*Inquiry

Teresa FazioColumbia Technology VenturesTel: (212) 854-8444Email: TechTransfer@columbia.edu

*IR

CU13153

*Principal Investigator

*Publications

J. Liu, M. Subir, K. Nguyen, K.B. Eisenthal. Second harmonic studies of ions crossing liposome membranes in real time. The Journal of Physical Chemistry B Letters. Vol. 112, 2008, pp.15263-15266.B. Doughty, S.W. Kazer, K.B. Eisenthal. Binding and cleavage of DNA with the restriction enzyme EcoR1 using time-resolved second harmonic generation. PNAS. Vol. 108, Issue 50, Dec. 2011, pp. 19979-19984.B. Doughty, Y. Rao, S.W. Kazer, S.J.J. Kwok, N.J. Turro, K.B. Eisenthal. Binding of the anti-cancer drug Daunomycin to DNA probed by second harmonic generation. The Journal of Physical Chemistry B. Article ASAP, Feb. 2013.B. Doughty, Y. Rao, S.W. Kazer, S.J.J. Kwok, N.J. Turro, K.B. Eisenthal. Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation. PNAS. Vol. 110, Issue 15, Apr. 2013, pp.5756-5758.

Country/Region

USA