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Heat Shock Protein 56 Monoclonal Antibody

Detailed Technology Description

Project ID:  D1996-05 Antigen Specificity: HSP56/FK506 binding protein 1A Clone: KN382/EC1 Specificity: Reacts with ~59 KDa FK506 binding protein (FKBP59/ FKBP52) Host species: Mouse Reactive species: Human, Rabbit, Mouse, Guinea pig, Hamster, Cow, Monkey, Mink  Description:This affinity purified antibody detects an ~59 kDa protein corresponding to the apparent molecular mass of FKBP59 on SDS-PAGE immunoblots in samples from human, monkey, bovine, mink, rabbit, hamster and guinea pig (weakly) origins. FK506 Binding Protein, FKBP59, was initially characterized as a component of steroid receptor complexes. FKBP59 has been identified as a member of the Hsp90/Hsp70 steroid receptor complex which has been used to differentiate between the active and inactive forms of steroid receptors. FKBP59 is alternatively termed FKBP52, HBI and Hsp56 in literature. FKBP59 is an immunophilin of the FK506 binding type whose cellular function is not fully known, but is thought to play a role in the folding and cytoplasmic/nuclear distribution of steroid receptors. FKBP59 (Hsp56) exhibits stress protein properties and has been shown to have increased expression in response to heat shock and ischemia.  Applications: Western blot, Immunohistochemistry, Immunoprecipitation References:1.     Nakao K, Myers JE, Faber LE. Development of a monoclonal antibody to the rabbit 8.5S uterine progestin receptor. Can J Biochem Cell Biol. 1985 Jan;63(1):33-40 [Link]2.     Sanchez ER, Faber LE, Henzel WJ, Pratt WB. The 56-59-kilodalton protein identified in untransformed steroid receptor complexes is a unique protein that exists in cytosol in a complex with both the 70- and 90-kilodalton heat shock proteins. Biochemistry. 1990 May 29;29(21):5145-52 [Link]3.     Sanchez ER. Hsp56: a novel heat shock protein associated with untransformed steroid receptor complexes. J Biol Chem. 1990 Dec 25;265(36):22067-70 [Link]4.     Gold BG, Densmore V, Shou W, Matzuk MM, Gordon HS. Immunophilin FK506-binding protein 52 (not FK506-binding protein 12) mediates the neurotrophic action of FK506. J Pharmacol Exp Ther. 1999 Jun;289(3):1202-10 [Link]

*Abstract

*Principal Investigator

Name: Lee Faber, Professor

Department: Physiology & Molecular Medicine

Country/Region

USA