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Purification of Small RNAs from Human Biofluids

Detailed Technology Description
This invention provides methods for isolating circulating small RNAs from plasma/urine, CSF, or tissue samples, the method comprising using an alkaline phenol:chloroform extraction, and methods of use
Countries
Not Available
Application No.
None
*Abstract

This invention provides methods for isolating circulating small RNAs from plasma/urine,CSF, or tissue samples, the method comprising using an alkaline phenol:chloroform extraction, and methods of use thereof, including for the detection, prognosis, and/or monitoring of disease in a subject. These methods significantly increase the yield of many assayable small RNAs such as miRNA, some by tenfold or more over present standard methods.

*IP Issue Date
None
*IP Type
Other Patent
Country/Region
USA

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