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Effective Chromosome Analysis with iChIP

Summary: To address these issues, researchers at Purdue University have developed immediate chromatin immunoprecipitaion (iChIP), a new, one-day procedure that significantly reduces the time and resources allocated to the detection of chromosome loci. This procedure reduces the time to approximately six hours or less and it greatly reduces the number of steps and reagents used in the process, making it highly cost-effective and efficient. The iChIP increases sensitivity, allowing for rapid screening of multiple loci and analysis of multiple targets that were previously difficult to detect. The iChIP would enable genomic localization of chromatin associated proteins and detection of histone modifications, i.e., K3K4 mono and trimethylation, and two yeast histone demethylases, i.e., Jhd2 and Rph1, which were previously difficult to detect when standard methods were used. A future potential use for the iChIP includes analyzing human cancer cell lines.

Technology Benefits: One-day analysis Reduced cost and processing steps Less complicated Increased sensitivity and rapid screening

Technology Application: Genetic R&D Pharmaceutical industry Medical/Health Future development of anticancer drugs

Detailed Technology Description: Scott BriggsPurdue BiochemistryPurdue Cell Identity and SignalingPurdue University Center for Cancer Research

Countries: United States

Application No.: None

*Abstract

*Background: Presently, genome wide chromatin immunoprecipitation (ChIP) studies have been used to detect multiple chromosome targets during research. The technologies that currently exist for the fast and standard detection of these loci, take two or three days or even a week to obtain relevant samples including an extra day for analysis.

*IP Issue Date: None

*IP Type: Utility

*Stage of Development: Prototype testing

*Web Links: Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipScott BriggsPurdue BiochemistryPurdue Cell Identity and SignalingPurdue University Center for Cancer Research

Country/Region: USA

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