Methods of Mutating, Modifying, Modulating Nucleic Acids in A Cell or Non-Human Mammal
- Detailed Technology Description
- None
- *Abstract
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Whitehead Intellectual Property Office is seeking a licensee for intellectual property relating to methods of mutating, modifying or modulating nucleic acids in a cell or nonhuman mammal. Genetically modified mice represent a crucial tool for understanding gene function in development and disease. It is costly and time-consuming to produce single gene knockout or knock-in mice, and even more so to make double mutant mice. Using novel approaches developed by scientists in the laboratory of Rudolf Jaenisch, a pioneer in stem cell research, mutant mice are generated by gene targeting using CRISPR/Cas9 in embryonic stem (ES) cells or zygotes and, if desired, precise genetic modifications are introduced through homologous recombination. The methods can produce homozygous mutant alleles or mutations in multiple genes with high efficiency. Gene-targeted ES cells injected into wild-type blastocysts can contribute to the germline of chimeric animals, and gene-targeted zygotes can be used to produce mice, thereby generating, in a single step, mice containing the targeted gene modification(s).
- Country/Region
- USA
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