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Narrow I.D. Monolithic Capillary Columns for High Efficiency Separation and High Sensitivity Analysis of Biomolecules

Technology Benefits

Value PropositionThe column/method:ΓÇóIs highly cost effective, as it avoids use of expensive equipments such as high pressure pumps and special adaptersΓÇóEnables a high efficiency separation and a high sensitivity analysis of biomolecules as compared to conventional approachesΓÇóAllows for analytes to have a higher electrospray ionization abilityΓÇóCompletely avoids band broadening problems as observed with prior art approachesΓÇóMinimizes the monolith structural irregularities as associated with capillaries narrower than 50 ┬╡m IDΓÇóHas an extremely small internal diameter of ~25┬╡m or less as compared to conventional columnsΓÇóExhibits enhanced mass transfer properties with a low back pressureΓÇóExhibits an excellent column-column reproducibility as compared to conventional approachesΓÇóAllows for the analysis of biomolecules as low as one attomoleΓÇóWould be commercially useful for the following applications:o High mass sensitivityo Single or multi-dimensional nano-LC analysis of biological or clinical sampleso Effective coupling to separation/analytical techniques such as ESI-MS, MALDI-MSo Sample desalting prior to MS

Detailed Technology Description

None

*Abstract

Currently available liquid chromatography columns have an internal diameter (ID) ranging from 75-100 ┬╡m. As a result, such columns are not feasible to analyze the limited amount of samples such as a tumor biopsy, immune precipitated proteins and laser captured micro dissected cells. Moreover, it is observed that narrow bore columns (<50 ┬╡m ID) are difficult to pack due to high pressure requirements. This approach discloses a novel, narrow ID monolithic capillary columns for an efficient separation and analysis, overcoming most prior art limitations.

*Principal Investigator

Name: Alexander Ivanov

Department:

Name: Barry Karger

Department:

Name: Li Zang

Department:

Country/Region

USA