Native SDS Page
- 詳細技術說明
- There is a great need for a robust method thatcombines the electrophoretic resolution of proteins with retention of theirfunctional behavior. The laboratory of Dr. David Petering has devised a newmethod of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)in which proteins can be well separated during electrophoresis as well asmaintain their native 3-dimensional conformations and functional activity.
- *Abstract
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There is a great need for a robust method thatcombines the electrophoretic resolution of proteins with retention of theirfunctional behavior. The laboratory of Dr. David Petering has devised a newmethod of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)in which proteins can be well separated during electrophoresis as well asmaintain their native 3-dimensional conformations and functional activity. Thisnative SDS-PAGE method will allow for important new experiments to be conductedin the proteomic field and a better understanding of proteins such as isolationfrom the gel of native proteins for further analysis, direct in-gel assay ofenzymatic activity, direct in-gel survey of binding activity of proteins withsmall molecules (e.g. drugs, toxic agents) and macromolecules (e.g. proteinbinding partners including antibodies, cognate DNA binding sites), andisolation of protein for subsequent mass spectral identification.
In traditional SDS-PAGE the proteins are wellseparated but are denatured such that the structure and function are no longeradequately maintained for carrying out further functional assays. The current commercial alternative, known asnative PAGE, enables researchers to maintain protein function and structure,but the technique leads to poor separation and smearing of protein duringelectrophoresis. Our easy to implement Native SDS-PAGE method has retained theenzymatic activity of a number of enzymes tested, and has also maintainedprotein complexes in a bound state. This new technology will allow for multiplenew applications in drug discovery, proteomics, protein therapeutics, andtoxicology.
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- 國家/地區
- 美國
