Chemiluminescence-Assisted Cell Endocytosis Kinetics Assay
Real-time evaluation Statistical quantification Fast and convenient process
Characterize and quantification of intracellular kinetics Drug delivery vector screening
UCLA researchers have developed a novel method to monitor the cell endocytosis kinetics using the bioluminescent reaction of firefly luciferase nanocapsules. A kinetic model is established via correlating the bioluminescence intensity to nanoparticle levels and is used to provide real-time quantification of cell internalization process. The bioluminescent platform provides a fast and convenient way to monitor internalization kinetics, also contains potential for drug delivery vector screening.
Background There has been a great interest to deliver molecules (e.g. proteins and genes) into cells, aiming to alter or restore certain function of the cells for applications such as therapeutics, gene editing, and cell reprograming. The majority of studies focus on extracellular concentration and final output, while leave the intracellular events as a black box, leading to treatment failure, poor efficacy and adverse effect. Therefore, quantitative description on the kinetics of intracellular delivery is essential to fundamentally understand biological process, strive rational design and optimal properties for drug development. Additional Technologies by these Inventors Tech ID/UC Case 29317/2018-361-0 Related Cases 2018-361-0
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