Method for Screening Delta Opioid Receptor Modulators

The receptor may be used as a diagnostic tool for conditions involving opioid receptors. The invention provides a screen against recombinant receptor agonists and antagonists. The invention provides a negative screen to verify if drugs are truly selective to the delta opioid receptor. The opioid receptor can be prepared using standard peptide synthesis methods.

Researchers in the laboratory of Dr. Christopher Evans have cloned and characterized the delta opioid receptor. The present invention utilizes a cell based assay to detect the levels of activation of opioid receptors in the presence or absence of candidate drugs. The present invention provides methods for screening agonists and antagonists of the delta opioid receptor, and can potentially play a large role in the development of targeted therapies directed to treat a host of ailments. .

Patent Number: US6265563B1
Application Number: US1995387707A
Inventor: Evans, Christopher J. | Keith, Duane E. | Edwards, Robert H. | Kaufman, Daniel
Priority Date: 13 Aug 1992
Priority Number: US6265563B1
Application Date: 13 Feb 1995
Publication Date: 24 Jul 2001
IPC Current: G01N003353 | A61K00317088 | A61K0039395 | A61K004500 | A61K004800 | A61P002504 | C07H002104 | C07K0014705 | C07K001472 | C12N000115 | C12N000119 | C12N000121 | C12N000510 | C12N001509 | C12N001512 | C12P002102 | C12P002104 | C12P002108 | C12Q000102
US Class: 5360235 | 4350691 | 4350701 | 4350711 | 4350712 | 4352523 | 43525411 | 4353201 | 435325 | 435365 | 435475 | 4352402
Assignee Applicant: The Regents of the University of California
Title: Opioid receptor genes
Usefulness: Opioid receptor genes
Summary: The nucleic acids may be used to recombinantly express kappa and mu opioid receptors in host cells. These cells may then be used in assays to identify modulators of the receptors activity that may be used, for example as analgesics or to combat the effects of opioid addiction. The nucleic acids and their complements may also be used as probe sequences to identify and characterize opioid receptor nucleic acids.
Novelty: Nucleic acids encoding mammalian kappa and mu opioid receptors, useful e.g. to identify substances for treating opioid addiction and/or useful as analgesics

生物醫學

DNA /基因工程

6265563

Background

Opioid receptors are abundant in the central and peripheral nervous system and are the targets of both opiate drugs and a family of endogenous opioid peptides. Seminal work carried out by Dr. Evans' research group at UCLA on this receptor has led to key insights in the field of neuropharmacology. To date, the delta opioid receptor has been implicated in various diseases including, but not limited to, pain, depression, neuroprotection, drug abuse and impulse control disorders. Moreover, on-going work has hinted at additional roles for this critical receptor. A method for screening potential modulators of the delta opioid receptors would provide unparalleled insight into the development of targeted therapies against this key target.

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• A Novel System for Measuring Protease Activity

Tech ID/UC Case

22617/1993-576-0

Related Cases

1993-576-0

美國

桌面版