Imaging Cells In Flow Cytometer Using Spatial-Temporal Transformation
This method provides a traditional flow cytometer with the added benefit of cell imaging capabilities.
The approach can be applied to retrofit traditional flow cytometers to become imaging flow cytometers at a minimum cost.
Disclosed are methods, devices and systems pertaining to imaging flow cytometry. The method of the invention uses mathematical algorithms and a specially designed spatial filter as the only hardware needed to give flow cytometers imaging capabilities. Instead of CCDs or any megapixel cameras found in many imaging systems, in the invention high quality images of fast moving cells in a flow cytometer are obtained using photomultiplier tube (PMT) detectors, thus obtaining high throughput in manners fully compatible with existing cytometers. Proof of concept has been achieved with demonstration of imaging for cells travelling at a velocity of 0.2 m/s in a microfluidic channel, corresponding to a throughput of approximately 1,000 cells per second.
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State Of Development Proof of concept has been achieved with demonstration of imaging for cells travelling at a velocity of 0.2 m/s in a microfluidic channel, corresponding to a throughput of approximately 1,000 cells per second. Intellectual Property Info This technology has a patent pending and is available for licensing. Related Materials Tech ID/UC Case 27502/2015-055-0 Related Cases 2015-055-0
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