Nitrate-Responsive Synthetic Promoter Produces Nitrate-Regulated Gene Expression in Plants
This promoter could be used in commercially relevant food crops to discover critical genes important to the productivity and survival of the plant.This synthetic NRP could also be utilized to drive transgene expression in GM crops in a nitrate inducible manner. Driving the expression of genes determining the efficiency of nitrogen utilization and other nitrate responsive pathways will allow for engineering increased efficiency of nitrogen utilization and improved crop productivity.Improved crop responsiveness to soil nitrate would lead to lower requirements for toxic fertilizers and reduce the impact of crop fertilization on contamination of the water and air.
UC San Diego investigators have developed a synthetic nitrate-inducible promoter (NRP), which is acutely responsive to nitrate levels in the soil. When fused to DNA encoding a protein or RNA and then inserted into transgenic Arabidopsis plants, this synthetic promoter will express the RNA or protein at levels in direct correlation with the amount of nitrate the plant is exposed to. This promoter has been successfully used to identify genes and elements important for nitrate responsiveness.
Patent Number: US20120210460A1
Application Number: US13392496A
Inventor: Crawford, Nigel | Wang, Rongchen | Guan, Peizhu | Chen, Mingsheng
Priority Date: 3 Sep 2009
Priority Number: US20120210460A1
Application Date: 7 May 2012
Publication Date: 16 Aug 2012
IPC Current: A01H000500 | A01H000106 | A01H000510 | C12N000121 | C12N000510 | C12N000701 | C12N001570 | C12N001582 | C12N001585
US Class: 800278 | 4352351 | 43525233 | 4353201 | 435325 | 435419 | 435455 | 435468 | 435471 | 800298
Assignee Applicant: The Regents of the University of California
Title: NITRATE-RESPONSIVE PROMOTER
Usefulness: NITRATE-RESPONSIVE PROMOTER
Summary: As expression cassette comprising nitrate responsive promoter, for introducing into cell or plant for inducing expression of the promoter, and for producing plant, plant part, seed or plant cell (claimed). The expression cassette is useful for identifying and isolating promoter and enhancer sequences that induce or repress genes regulated by nitrate, such nitrogen fertilizers i.e. for screening nitrate enhancers in transgenic plants. The nucleic acids of expression cassette are useful to confer desired traits on plants including plant species from genera Asparagus , Atropa , Avena , Brassica , Citrus , Citrullus , Capsicum , Cucumis , Cucurbita , Daucus , Fragaria , Glycine , Gossypium , Helianthus , Heterocallis , Hordeum , Hyoscyamus , Lactuca , Linum , Lolium , Lycopersicon , Malus , Manihot , Majorana , Medicago , Nicotiana , Oryza , Panieum , Pannesetum , Persea , Pisum , Pyrus , Prunus , Raphanus , Secale , Senecio , Sinapis , Solanum , Sorghum , Trigonella , Triticum , Vitis , Vigna and Zea .
Novelty: New expression cassette for inducing expression of nitrate responsive promoter in plant comprises promoter having specified nucleic acid sequence, operably linked to heterologous polynucleotide, linked to minimal promoter sequence
農業
植物
8927807
Intellectual Property Info This technology is available for licensing in the U.S. A commercial sponsor for product development is sought. Related Materials Tech ID/UC Case 19949/2010-073-0 Related Cases 2010-073-0
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