Monoclonal Antibody Against PNPase (Clone 3H5)
Specific antibody to detect mtPAP (clone 1D3) by antibody-antigen complex formation.
Immunocytochemistry/immunofluorescence (1:100-1:1000)* Immunoprecipitation Western blot * Suggested dilutions
RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonuclease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. The described mouse monoclonal antibody binds to human mtPAP and has been described for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.
Related Materials Additional Technologies by these Inventors Tech ID/UC Case 25121/2011-375-0 Related Cases 2011-375-0, 2011-387-0, 2011-386-0, 2011-385-0, 2011-384-0, 2011-383-0, 2011-382-0
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