Peptide that captures and anchors thiol-reactive molecules to measure hypoxia
Biochemistry techniques such as enzyme-linked immunosorbent assay (ELISA) are very useful for determining the amount of specific antigens or antibodies in a given sample. Some important chemical species, such as thiol-reactive molecules, can be particularly difficult to assay. This technology utilizes a cost effective peptide sequence that displays a high affinity for both the plastic of a multi-well or nanofabricated flowcell device and proteins known to interact with thiols such as those in hypoxic environments. This ELISA-based assay can quickly detect the efficacy of tumor hypoxia-targeted drugs for rapid screening of new compounds or to predict patient-specific efficacy of therapeutics targeting solid tumors.
Cheaper than alternativesFastSimplePatent Information:Patent Pending (WO/2016/140860)Tech Ventures Reference: IR CU15135
Determining amounts of thiol-reactive molecules in samplesDetermining the efficacy of tumor hypoxia therapiesAffinity columns for separation
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