Single Molecule Sensor on Nanopore platform for Ion Channel Studies
Kenneth Shepard, Ph.D.
High bandwidth sensingHigh throughput detection through multiplexingFlexible design configurations for broad range of applicationsPatent information:Patent Pending
High Throughput Single Molecule Sensing with Improved Sensitivity and Design Flexibility for Broad Suitability for in vitro and in situ ApplicationsHigh resolution sensing of single molecules has been hitherto limited by background noise in the nanopore, with a maximal measurable bandwidth of 100kHz. By reducing the parasitic capacitance of the system, this technology improves that bandwidth to at least 1 MHz thus improving the sensitivity. This technology utilizes low noise amplifiers, which have significantly lower parasitic capacitances than traditional external amplifier platforms. Additionally, parasitic capacitance arising from unfavorable interactions with the ionic electrolytes is reduced by protectively coating the nanopore membrane and amplifier surface. Lastly, the electrode is integrated closer to the amplifier, at a distance of 100 microns compared to the typical 10 centimeters to reduce parasitic capacitance. The planar amplifier design of this CMOS nanopore platform also lends itself to the possibility of parallel detection schemes. This technology is equipped with multiple pre-amplifiers in parallel for multiplexed ionic channels measurement. This multiple detection scheme in conduction with higher resolution sensing can yield high throughput measurements that could be useful for commercial applications. This CMOS Nanopore platform is able to incorporate various design configurations for increased flexibility. A range of interface designs are specified in this technology to accommodate a wide range of in vitro and in situ applications. High throughput nanopore sensorsHigh throughput DNA sequencingDrug discovery and screening efficiencyBiocompatible implantable sensorsChemical and environmental sensorsIon channel measurements and kinetic studies
Kenneth Shepard, Ph.D.
美國
