Tissue Preservation via Induced Synthesis of Heat Shock Protein HSP 70
Lead Inventors: Henry N. Ginsberg, M.D.Problem or Unmet Need:Organs or cells removed from a donor are susceptible to injury due to the effects of noxious stimuli or stressful conditions such as heat shock (i.e., an abrupt increase in temperature), hypoxia, and ischemia. There is a need to protect ex vivo tissue from these effects in order maintain tissue viability for therapeutic purposes, e.g., transplantation. The technology is a novel method for increasing the levels of heat shock protein HSP 70 in a cell. This protein (which comprises the proteins HSP 72 and HSP 73, only the former of which is inducible by heat and other forms of stress) serves as a molecular chaperone that protect proteins from damage due to misfolding or unfolding when a cell is subjected to heat shock. This method relies upon the observation that the aldehydic tripeptide acetyl-leucyl-leucyl-norleucinal (ALLN) both stabilizes HSP 70 levels by inhibiting a protease that normally degrades HSP 70 and dramatically increases synthesis of HSP 70.
Using ALLN to increase HSP 70 levels is a natural way to improve cellular response to injury and/or stressful conditions.
Can potentially be used to better preserve an organ ex vivo by protecting it from noxious stimuli or stress. Could greatly facilitate organ transplantation.
The technology is a novel method for increasing the levels of heat shock protein HSP 70 in a cell. This protein (which comprises the proteins HSP 72 and HSP 73, only the former of which is inducible by heat and other forms of stress) serves as ...
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