kHz Frame Rate Confocal Imaging
Purdue University researchers have designed a confocal microscope capable of capturing quantitative images with a frame rate on the scale of kHz, which is faster than the 30 Hz for current microscopy techniques. As opposed to the rectangular scan pattern used in other microscopes, the Purdue researchers' instrument captures pixels along a Lissajous curve whose geometry can be tuned by the user. This scan pattern requires much less time to capture an image. By coupling this scan pattern with image processing software, the potential for this approach is unlocked. For any given frame of a movie, the dark spots within the scan pattern can be statistically interpolated from the scanned pixels and the movement of the image over time. If a high-resolution still image is preferred, then individual frames are combined, like those in a panoramic photograph, to assemble the high-resolution image. In this way, both low-resolution movies and high-resolution stills can be captured from the same data set. This combination of Lissajous scan-pattern and software processing shows promise for faster detection of microscopic processes and is flexible enough to be extended for long-distance applications like lidar.
Much faster frame rate than current confocal microscopesRaw data can be used to generate high-resolution still imageGeneration of a high-frame rate, lower-resolution movie
Microscopy applications where movement distortion is a problemMicroscope use in exotic environmentsLidar telescopesScanned laser imaging
Garth SimpsonSimpson Laboratory for Nonlinear OpticsPurdue Chemistry
United States
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美國
