Method and system for detection of chloramphenicol
Fast and simple method of detecting the presence of chloramphenicol, a harmful compound if present in food products. The method makes use of a mutant chloramphenicol acetyltransferase (CAT) and a fluorophore-linked chloramphenicol in a system where chloramphenicol and the fluorophore-linked chloramphenicol competes for the active site of the mutant CAT. Because the fluorophore-linked chloramphenicol reduces its fluorescence upon binding to the active site and vice versa increases its fluorescence upon being displaced from the active site by the presence of unmodified chloramphenicol in a sample, the increase of fluorescence caused by a testing sample indicates the presence of chloramphenicol.
Patent Number: US8329425B2
Application Number: US13036021A
Inventor: Wong, Kwok-Yin | Leung, Yun Chung | Chan, Pak Ho | Chung, Wai Hong | Chow, Ka Yan | Chow, Ho yin | Leung, Hon Man
Priority Date: 28 Feb 2011
Priority Number: US8329425B2
Application Date: 28 Feb 2011
Publication Date: 11 Dec 2012
IPC Current: C12Q000148 | C12N000910
US Class: 435015 | 435193
Assignee Applicant: The Hong Kong Polytechnic University
Title: Method and system for detection of chloramphenicol
Usefulness: Method and system for detection of chloramphenicol
Summary: The method is useful for quantitative or qualitative measuring chloramphenicol in a sample (claimed), preferably food products.
Novelty: Method for quantitative or qualitative measuring chloramphenicol, comprises e.g. creating mutant chloramphenicol acetyltransferase, creating fluorophore-linked chloramphenicol ligand, and bringing fluorophore-linked chloramphenicol ligand
診斷/治療
其他疾病
Wong Kwok Yin
Leung Yun Chung, Thomas
Chan Pak Ho
Chung wai Hong
Chow Ka Yan
Chow Ho Yin
LEUNG Hon Man
美國
