Recombinant bacillus proteases and uses thereof
The present invention provides genetically engineered Bacillus strains that can secrete large amount of Bacillus proteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules of Bacillus origin in a Bacillus subtilis strain 168, utilizing a strong prophage promoter.
Patent Number: US7081359B2
Application Number: US2002146905A
Inventor: Lim, Boon Leong
Priority Date: 17 May 2002
Priority Number: US7081359B2
Application Date: 17 May 2002
Publication Date: 25 Jul 2006
IPC Current: C12P002106 | C07H002104 | C12N000121 | C12N000950 | C12N000954 | C12N001500 | C12N001575
US Class: 435219 | 4350691 | 435221 | 435222 | 43525231 | 4353201 | 5360232 | 5360241
Assignee Applicant: Enzymes Technology Limited,Kwai Chung
Title: Recombinant bacillus proteases and uses thereof
Usefulness: Recombinant bacillus proteases and uses thereof
Summary: The method is useful for producing Bacillus protease (claimed) for applications in commercial processes, e.g., detergent application.
Novelty: Producing Bacillus protease by transforming a recombinant Bacillus strain with an expression vector comprising a promoter region of the ORF19 of the bacteriophage phi-105 and a gene from Bacillus strains encoding the protease
生物醫學
DNA /基因工程
香港
