Fluorescent Water-Soluble Conjugated Polyene Compounds That Exhibit Aggregation Induced Emission and Methods of Making and Using Same具有聚集誘導發光特性的多烯類螢光生物探針基于四苯基乙烯染料的水分散性螢光高分子微納米球
In the present invention we designed and synthesized a series of water-soluble linear and cyclic pi-conjugated organic compounds (hereinafter polyenes) with different chromophores including tetraphenylethylene, siloles, fulvene, butadienes, and 4H-pyrans. The emission color of these polyenes ranges from blue to red arising from the different chromophoric structures. Their fluorescent behavior features the aggregation-induced emission (AIE) phenomenon. When dissolved in aqueous media, they are practically nonemissive, however, the fluorescence can be turned on in the presence of biomacromolecules such as proteins, DNA and RNA. A linear relationship between the fluorescence intensity and the concentration of analytes is also shown in a certain range, which is of great importance for protein and DNA assays. All these features make AIE-active molecules excellent candidates for the usage of various sensor and detection devices.
1. Highly sensitive to biomacromolecules
2. Water-solubility
3. Stable
4. Easily prepared and processed into a device
5. Easily decorated with any functional groups or reactive sites, such as oligonucleotide, antibody, etc.
- The invented materials might find applications as different kinds of biosensors, in particular for the DNA and protein assays.
Patent Number: US7939613B2
Application Number: US2007130A
Inventor: Tang, Benzhong | Hong, Yuning | Haeussler, Matthias | Tong, Hui | Dong, Yongqiang | Li, Zhen | Xing, Changmin
Priority Date: 22 Apr 2005
Priority Number: US7939613B2
Application Date: 10 Dec 2007
Publication Date: 10 May 2011
IPC Current: C08F022012 | C12Q000168 | C12Q000170 | G01N001506 | G01N003353
US Class: 5263291 | 4220681 | 435005 | 4350061 | 4350071 | 4350072 | 435006
Assignee Applicant: The Hong Kong University of Science & Technology
Title: Fluorescent water-soluble conjugated polyene compounds that exhibit aggregation induced emission and methods of making and using same
Usefulness: Fluorescent water-soluble conjugated polyene compounds that exhibit aggregation induced emission and methods of making and using same
Summary: (A) are useful in sensor device for detecting the presence or absence of target bio-macromolecule in biological sample, which is a tissue sample, cell sample, blood, saliva, spinal fluid, lymph fluid, vaginal fluid, seminal fluid or urine. (A) are useful to make the polymeric particle, which is useful in formulation comprising a bio-probe, coating, paint, flexible free-standing film, cosmetic, fluidic tracer, or a marker (all claimed), where the fluidic tracer is useful to investigate capillary flow, define neuronal cell connectivity, study dye translocation through gap junctions, and to follow cell division, cell lysis or liposome fusion, and the marker is useful as an indicator of a biologic state e.g. pH, polarity, and viscosity of the biological environment. (A) are useful as "turn-on" fluorescence sensors.
Novelty: New conjugated polyene compounds, useful e.g. in sensor device for detecting the presence or absence of target bio-macromolecule in biological sample e.g. tissue sample, blood, saliva, spinal fluid, vaginal fluid, seminal fluid or urine
生物醫學
DNA /基因工程
10 Dec 2007
US 12/000130
US 7939613
TTC.PA.324S, TTC.PA.339
香港
