Dual-Enzyme Responsive Peptides
Allows much greater selectivity and specificity Better-controlled degradation of opioid formulations
Enzyme-responsive systems for diagnostic and drug delivery applications Selective biodegradation of materials for biomedical applications (i.e. location-specific degradation and drug release for abuse-deterrent opioid formulations)
Researchers at UCLA have designed a dual-enzyme responsive peptide system that requires sequential digestion by two enzymes for cargo release from the C-terminus. In this system, the peptide is first cleaved by an enzyme that unmasks the recognition site for a second enzyme, allowing for digestion and release of the final product. These peptides can be used in polymeric formulations, either as cross-linkers or incorporated into the backbone, installing dual-enzyme sensitivity. This method may also be useful for delayed release formulations/prodrugs, allowing degradation of abuse-deterrent opioid formulations to be better controlled when sequences that require digestions by multiple enzymes are installed.
State Of Development Have demonstrated sequences that are responsive to trypsin/chymotrypsin, trypsin/papain, and trypsin/caspase 3. Background Due to their high selectivity and specificity, enzyme responsive systems are commonly used for diagnostic and drug delivery applications. Currently, most enzyme responsive technologies are sensitive to a single enzyme, or a single enzyme in combination with an environmental stimulus. For example, caspase-sensitive reporters, which respond to peroxide production or to cancer-related matrix metalloproteinases, have been designated to detect cell injury as well as to monitor reactivation of the apoptotic pathway after anti-cancer therapy delivery. Trypsin responsive sequences have been incorporated into abuse-deterrent opioid formulations, which allow drug release only at specific locations in vivo. Although many single-enzyme responsive systems show promise in specific targeting, response to more than one enzyme would allow for greater target selectivity and indirect enzyme detection, and provide information about cellular environments. Additional Technologies by these Inventors Tech ID/UC Case 29379/2017-446-0 Related Cases 2017-446-0
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