Anti-Phytochelatin Synthase Monoclonal Antibodies (3 lines)
These mouse IgG2a monoclonal antibodies were generatedagainst synthetic peptide and recognize plants, fission yeast (Schizosaccharomyces pombe) and itsclosely-related organisms’ phytochelatin synthetase (PS), N-MAP. All lines havebeen validated for use in WB, ELISA, and IF. Together with gamma-glutamyl Cys sythetase (γ-ECS) andglutathione synthetase, phytochelatin synthetase (PS) is an enzyme involved in,and required for, synthetizing phytochelatins (PC). In certain plants,deficiency of such enzymes leads to low tolerance to metal contamination whilemutants over-expressing them are highly tolerant to metal contamination and maybe used in phyto-remediation of heavy metals. Monoclonal antibodies to PC weredeveloped using N-terminal peptides synthesized as MAPs. N-terminal MAP was 29amino acids long. They are useful for the study of synthesis and development ofphytochelatins and related metabolic pathways, as well as in the development ofphytoremediation tools and in the study of metal toxicity in plants and closelyrelated organisms.
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