CELL-BASED OVER-EXPRESSION OF CARBOXYLIC ACIDS AND AMINO ACIDS OF INDUSTRIAL INTEREST
ApplicationGeneticengineering of cells of essentially any organism (mammal, bacteria, yeast,fungi, etc.) for the over-production of carboxylic acids and amino acids producedin the TCA cycle. ProblemsAddressed (benefits/advantages)Production of oxaloacetate-derived commodity chemicals viabiofermentation is subject to the stringent metabolic regulation of carbon flowin the TCA cycle which can limit process yields. Carbon flux towards oxaloacetate(OAA) remains constant regardless of system perturbations. In typicalprocesses, production of OAA remainsconstant and product yields are relatively low. Hence, a tremendous opportunityexists to improve the process by overcoming the metabolic regulation of carbonflow. Anaerobic fermentation processes can be used to produce oxaloacetate-derivedorganic acids such as malate, fumarate, and succinate, as well as amino acidsand their derivatives.
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