Method to Expand and Transduce Cultured Human Small and Large Intestinal Stem Cells

Rapid expansion of intestinal stem cells in culture without differentiationAlso permits expansion of stem cells from polyposis disorders, and likely cancer cellsDoes not require a feeder layerResulting tissue can be implanted into humansEfficient genetic manipulation of human cells

Use to rapidly expand human small and large intestinal mucosal cells for basic research, therapeutic or toxicological studiesGenetic modification of intestinal stem cells in order to identify important genes Differentiation of intestinal stem cells into implantable tissue as a treatment for intestinal

Dr. Martin G. Martin and colleagues have developed novel methods of expanding, differentiating and genetically modifying human small and large intestinal stem cells. Isolated human intestinal stem cells and epithelium have been successfully expanded in culture using a combination of specific conditioned media and inhibitors in order to allow for expansion. The media conditions can then be modified to allow cells to differentiate into mature cell types. Unlike previous methods, this method does not require a feeder layer, and can therefore be transferred into humans once differentiated. Stem cells and epithelium grown in this manner have been successfully transduced with lentiviral vectors, which allows for a range of genetic manipulation including gene-specific knockdown and overexpression.

20160068805

State Of Development

• Method shown to be robust and reproducible in vitro
• Investigators are in the process of identifying more specific factors for growth

Background

Stem cells are early progenitor cells that have the potential to self-renew through cell division or to differentiate into specialized mature cell types. Small and large intestine stem cells are somatic stem cells capable of differentiating into all five-cell types found in mature intestine. Ideally, these cells would be expanded in culture, where they could be expanded and genetically manipulated, and then differentiated into mature tissue and transplanted into patients. In order to properly expand stem cells in culture, techniques must be found to expand the cells while maintaining the stem cell qualities that allow them to differentiate into various mature cell types. Current methods of human small and large intestinal stem cell culture have been difficult to reproduce and have a limited capacity to expand in vitro. Therefore, new methods to expand, differentiate and genetically modify human small and large intestinal stem cells are needed.

Tech ID/UC Case

24160/2013-672-0

Related Cases

2013-672-0

美国

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