Kit for Identification of Traces of High Explosives Using Thin Layer Chromatography
UC San Diego researchers have developed sensors, sensor fabrication processes, and sensing methods that enable detecting traces of high explosives by fluorescence quenching in combination with chromatographic separation. The invention includes use of fluorescent, silicon based copolymers, covalently linked to silica thin-layer chromatographic plates or nanoparticle supports. A thin layer of the polymer allows for a more efficient interaction with an analyte, thus yielding enhanced detection sensitivity. The attachment of the sensing polymers onto a chromatographic support allows for the separation of a mixture, as well as the identification of multiple explosives through the use of multiple sensing polymers. Coating silica nanoparticles with the sensing polymer provides a simple means of detecting high explosives in aqueous media with a low detection limit. The invention can be implemented as inexpensive field-ready kits for the identification of trace explosives.
Inventor: Zhang, Linqi | Lewin, Sharon R. | Kostrikis, Leondios | Ho, David D.
Priority Number: US6235504B1
IPC Current: C12Q000168 | C12Q000170
US Class: 4350912 | 4350911 | 5360231 | 5360243 | 53602431 | 53602432 | 53602433 | 53602532
Assignee Applicant: The Rockefeller University,New York
Title: Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
Usefulness: Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
Summary: The method is useful for determining the number of copies per cell of at least one preselected polynucleotide target sequence in a nucleic acid sample of cellular origin. The method is specifically useful in monitoring T cell receptor circle sequences, where the method provides an indication of the recovery of the immune system with various therapies; in monitoring thymic function with age; and in quantifying viral, microbial or cellular sequences. The genomic equivalent marker may be used to determine the number of copies of a marker of thymocyte proliferation, to identify other genetic sequences for use as genomic equivalent markers, and to facilitate denotation of target cellular nucleic acid levels as abundance per cell;
Novelty: Determining the number of copies per cell of a preselected polynucleotide target sequence, for monitoring immune system recovery, comprises quantifying at least one target sequence and at least one genomic equivalent marker sequence
生物医学
DNA /基因工程
9134239
State Of Development This technology has a working prototype. Intellectual Property Info The invention has a patent pending and is available for licensing. Related Materials Hollow Silica Nanospheres Containing a Silafluorene-Fluorene Conjugated Polymer for Aqueous TNT and RDX Detection, Chem. Commun., 2010, 46, 6804-6806. Tech ID/UC Case 21905/2011-061-0 Related Cases 2011-061-0
Luminescent Organosilicon Polymers and Sol-Gel Synthesis of Nano-Structured Silica, Martinez, H. Paul, Ph.D., Dissertation, University of California, San Diego, 2011.
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