Method For High Level Production Of Recombinant Protein
The present invention provides methods for delivering higher yields than the Baker system. The invention is useful for the production of both soluble and membrane proteins. The technology produces proteins and peptides with authentic N termini for a range of downstream applications. Commercial applications include reagent uses, such as: Production of proteins in their native form Single or multiple amino acid mutants Isotopically labeled reagents Fluorescent or biotinylated reagents Reagents for protein therapeutics Animal testing reagents and/or components of kits
Scientists at the UC San Diego have designed a recombinant protein expression system that delivers significantly higher yields than previously reported systems and includes an easy means of purification. It allows the production of a variety of proteins and peptides with authentic N termini for a range of downstream applications. Higher yields are obtained by redesigned protein core amino acids because of reduced toxicity and proteolysis due to the "tuned" stability of the ubiquitin fusion. They are expressed in inclusion bodies that enable the proteins to be refolded and efficiently cleaved from the fusion by ubiquitinase. This results in higher expression levels than comparable systems that express soluble proteins. Ultimately, the present technology can be useful for the production of both soluble and membrane proteins.
Inventor: HANDEL, Tracy, M. | DOUGLAS, Andrew
Priority Number: WO2012129449A3
IPC Current: C07K001900 | C12N001562 | C12N001570
Assignee Applicant: The Regents of the University of California
Title: HIGH LEVEL PRODUCTION OF RECOMBINANT PROTEINS | PRODUCTION À HAUT NIVEAU DE PROTÉINES RECOMBINANTES
Usefulness: HIGH LEVEL PRODUCTION OF RECOMBINANT PROTEINS | PRODUCTION À HAUT NIVEAU DE PROTÉINES RECOMBINANTES
Summary: The method is used for producing a recombinant protein (claimed).
化工/材料
化工/材料应用
State Of Development A U.S. patent application has been filed. Tech ID/UC Case 19155/2009-147-0 Related Cases 2009-147-0
美国
