Continuous, enhanced detection of droplet contents in electrical impedance spectroscopy
Continuous amplified detection in separate droplets Does not require fluorescent dye
Label-free detection of DNA Nanoparticle-enhanced detection of droplet contents
Amplified detection of DNA in droplets has been demonstrated using a fluorescent probe. The use of fluorescent markers is expensive and requires extra processing steps. In addition, fluorescence intensity results must be compared to a calibration curve. Electrical impedance spectroscopy (EIS) has been used for the detection of flowing whole cells or static subcellular components. The inventors at UCI have developed a method and system that allows the detection of a gene in genomic DNA and provides a platform that can detect DNA in flowing droplets. The technique incorporates molecular affinity forces to align molecules within droplets at the interface, resulting in increased EIS sensitivity. The method also allows for continuous measurements in traveling microfluidic droplets. The process is ideal for entities using polymerase chain reaction (PCR), as the technique is label-free and provides the capability to detect PCR amplification in many samples in real time.
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State Of Development The system for both continuous microfluidic droplet generation and electrical impedance spectroscopy measurements has been developed and tested. Tech ID/UC Case 27049/2014-743-0 Related Cases 2014-743-0
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