Inline-injection microdevice and microfabricated integrated DNA analysis system
Eliminates cross-injection geometries, excess sample requirements, and delicate timing sequences inherent in cross-injection systemsProduces high quality Sanger separations from only 100 attomoles of templatePermits Sanger sequencing miniaturization at a level limited only by detector sensitivity
DNA or RNA SequencingCapillary gel electrophoresis
None
Patent Number: US20090035770A1
Application Number: US2007978224A
Inventor: Mathies, Richard A. | Blazej, Robert | Kumaresan, Palani
Priority Date: 25 Oct 2006
Priority Number: US20090035770A1
Application Date: 25 Oct 2007
Publication Date: 5 Feb 2009
IPC Current: C12Q000168 | B01D005702 | B01L009900 | C12M000134 | C12M000140
US Class: 43500619 | 435006 | 204451 | 204453 | 204604 | 4352873 | 4352891
Assignee Applicant: The Regents of the University of California
Title: Inline-injection microdevice and microfabricated integrated DNA analysis system using same
Usefulness: Inline-injection microdevice and microfabricated integrated DNA analysis system using same
Summary: As a microfabricated device for inline injection of a sample plug into a separation channel; useful for introducing an analyte in a sample to a separation channel, and for detecting resolved analyte molecules in separation channel; useful for paired-end sequencing; useful in a microfabricated system for performing sequencing e.g. DNA sequencing (claimed) and RNA sequencing, preferably via capillary gel electrophoresis, and for performing polymerase chain reactions (PCR); useful for inline injection of nucleic acids for capillary electrophoresis analysis; and useful in micro-fabricated integrated DNA analysis system.
Novelty: Microfabricated inline-injection device useful in microfabricated integrated DNA analysis system, comprises sample channel region; and capture channel region arranged inline with separation channel region
生物医学
DNA /基因工程
8841116
Brief Description The cross-injector system is utilized in nearly all microfabricated capillary electrophoresis applications to form small, well-defined sample plugs. Using the cross-injector system, however, an excess sample volume is necessary to form and inject the sample plug. In addition, without desalting, the sample plug will contain ionic buffer and other ionic reagents. Because of these factors, cross-injector systems have efficiencies of 1% or less. In advanced integrated microdevice systems that seek to miniaturize not only CE, but also all processing steps, the cross-injector is a barrier to reaching theoretical miniaturization limits. Additionally, cross-injection timing requirements can hinder optimization of array CE microdevices and integrated bioprocessor systems operating on limiting amounts of template. T-injection designs also present requirements that have similar barriers to miniaturization and timing optimization. Direct analyte injection that has been used in certain CE applications yields low-resolution, low-sensitivity separations due to the large sample plug size and injection of contaminants that are not suitable for certain applications. UC Scientists have described methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis that solve the problems described above. The microfabricated structures include affinity-based capture matrixes inline with separation channels. The affinity-based capture matrices provide inline sample plug formation and injection into a capillary electrophoresis channel. Also described are methods and apparatus for microbead-based inline injection system for DNA sequencing. Publications Inline Injection Microdevice for Attomole-Scale Sanger DNA Sequencing Tech ID/UC Case 17766/2007-042-0 Related Cases 2007-042-0
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